NRF2 AND HSP70 SYNERGIC EFFECT IN OXIDATIVE STRESS MODULATION DURING HK-2 CELL MECHANICAL DEFORMATION

MARTÍN E RINALDI TOSI; VICTORIA BOCANEGRA; WALTER MANUCHA; LUCÍA FUENTES; PATRICIA G. VALLES

Potrero de los Funes, San Luis

Jornada; XXX Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2012

Sociedad de Biología de Cuyo

Cellular mechanical deformation is characteristic of hydronephrosis during obstructive nephropathy. Cellular stretching induces NADPH oxidase activity (NOX-ac) and oxidative stress. The target was to evaluate mechanical deformation in human kidney cells (HK- 2) at different periods of time and to assess cytoprotective effects of both Hsp70 and Nrf2 pathways. Oxidative stress was demonstrated by total antioxidant status (TAS) and NOX-ac assays, Hsp70 expression by Western-blot and GSTA2 induction by Nrf2, through RT-PCR. The increased NOX-ac and the fall off TAS contributed to oxidative stress after 1 and or 2 hours of mechanical deformation, (p