IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
HISTOLOGICAL AND MOLECULAR STUDY DURING OBSTRUCTIVE NEPHROPATHY: PARICALCITOL EFFECTS AT MITOCHONDRIAL LEVEL
Autor/es:
GARCÍA IM; MAZZEI L; ALTAMIRANO L; MOLINA M; FERDER L; , DIEZ E; PONCE ZUMINO ; OLIVEROS; FORNÉS MW; RINALDI TOSI M ; MANUCHA W
Lugar:
MENDOZA
Reunión:
Congreso; XXVIII Reunión Científica Anual Sociedad de Biología de Cuyo; 2010
Institución organizadora:
SOCIEDAD DE BIOLOGIA DE CUYO
Resumen:
New evidence suggests that the activators of Vitamin D receptors have a suppressant effect on oxidative stress. This study investigates, in an obstructive nephropathy (ON) animal model, the structural and functional changes in the kidney and the paricalcitol (Pari) effects. Methods: Ten adult female Wistar rats were obstructed surgically at the urethero-pelvic level. They were divided into two groups, Control and Treated. The treatment was done for a 15-day (30 ng/Kg/ day). We evaluated blood pressure, PTH, as well as calcium and phosphorous levels. The kidney was also evaluated histologically for fibrosis with Masson trichomic staining, for apoptosis with TUNEL technique, and electronic microscopy (EM) for mitochondrial evaluation. Results: Histological changes in the kidney showed that the ON induced interstitial fibrosis with increase in apoptosis. Both results decreased with Pari. EM revealed, in obstructed nontreated animals, electronically luminous nuclear material in the nucleus. We also noted that the mitochondria were increased in size with dilated crests and spaces in their interior. These mitochondrial changes were not present in the paricalcitol-treated animals. Conclusions: These results, in an obstructed nephropathy model, show a cyto-protected effect of the activator of Vitamin D receptors, paricalcitol, revealing for the first time a possible protective effect at the mitochondrial level.