Long-term effects of maternal mild hyperthyroidism on the development and the behavior of offspring in adulthood

SÁNCHEZ MARÍA BELÉN; SOAJE MARTA; JAHN GRACIELA ALMA; MICHEL LARA, MARÍA CECILIA; VIRUEL LUCIANA BELÉN; GOMEZ SILVINA; VALDEZ SUSANA RUTH; NEIRA FLAVIA JUDITH; PIETROBON ELISA OLIVIA; MACKERN OBERTI JUAN PABLO

Mendoza

Congreso; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo.; 2022

Sociedad de Biología de Cuyo

Abnormal levels of thyroid hormones (THs) are associated with alterations in anxiety and circulating hormones such as glucocorticoids (GCs) and prolactin affecting fertility and reproductive success. However, little is known about the long-term effects of maternalhyperthyroidism on the development and behavior of their offspring. For this purpose, eight pregnant Wistar rats were divided in two groups defined as: Control (Euthyroid, n=4) and HyperT (n=4). Hyperthyroidism was induced with T 4 0.1mg/kg/day, s.c. After delivery,on day 2 of lactation, the number of pups in each litter was standardized to eight and the offspring allowed to growth under standard condition according to the NIH guide for the Care and Use of Laboratory Animals. Pup development parameters were recorded from birth to 42 postnatal day (PND). Adult female and male offspring (PND 85-100) were subjected to the Open Field Test (OFT) to evaluate locomotor activity using EthoWatcher computacional tool. The parameters assessed were as follows: a) rearings, b) entries to areas of interest, c) total distance traveled. At PND 100-120, the adult offspring were sacrificed, trunk blood was collected for serum hormonaldeterminations for ELISA and adrenal glands (AG) dissected for histological analysis using Image J program. We found that offspring from HyperT mothers (named HyperT pups) presented alterations in the postnatal development of some physical, sensory, motor, and l pups). Body length at PND 1 was lower in HyperT pups (p