IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
artículos
Título:
Anandamide-nanoformulation obtained by electrospraying for cardiovascular therapy
Autor/es:
MARTÍN GIMÉNEZ, VIRNA MARGARITA; VIVERO-LOPEZ, MARÍA; PRADO, NATALIA; MANUCHA, WALTER; DÍAZ-RODRÍGUEZ, PATRICIA; CONCHEIRO, ANGEL; ENRIQUE KASSUHA, DIEGO; SANZ, RAÚL LELIO; DIEZ, EMILIANO; ALVAREZ-LORENZO, CARMEN
Revista:
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2019 vol. 566 p. 1 - 10
ISSN:
0378-5173
Resumen:
Anandamide (AEA), an endogenous cannabinoid, has a relevant antihypertensive effect. However, its cardioprotective role has been barely explored due to unfavorable physico-chemical properties and, sometimes, undesirable psychoactive effects. In this context, drug encapsulation in nanocarriers could overcome the limitations associated with the administration of AEA in free form. The aim of the present study was to encapsulate AEA in poly-ε-caprolactone/Pluronic® F127 nanoparticles (AEA/PCL/PF127 NPs) by means of electrospraying, to characterize their physico-chemical properties and cytocompatibility and to evaluate their effect in an in vivo model of cardiovascular remodeling caused by hypertension. AEA/PCL/PF127 NPs were characterized in terms of morphology, size, polydispersity, Z-potential, hydrophilicity, thermal and spectroscopic properties. Also, the encapsulation and loading efficiencies and in vitro release of AEA were analyzed. AEA/PCL/PF127 NPs (700-1000 nm) showed adequate cytocompatibility. For the cardiovascular remodeling studies, normotensive (WKY) and hypertensive (SHR) male rats were treated or not with AEA/PCL/PF127 NPs (5 mg/Kg, intraperitoneal injection) weekly for 1 month. Inflammatory markers and hemodynamic, structural and cardiac functional parameters were monitored. In SHR, the treatment with AEA/PCL/PF127 NPs reversed all altered cardiovascular markers and parameters (p