IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
artículos
Título:
VASCULAR REPAIR BY ENDOTHELIAL PROGENITOR CELLS IN AN EXPERIMENTAL MODEL OF METABOLIC SYNDROME
Autor/es:
RENNA NF; LEMBO, C; VAZQUEZ-PRIETO MA; LAMA C; GONZALEZ S; MIATELLO RM
Revista:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
Editorial:
AMER PHYSIOLOGICAL SOC
Referencias:
Año: 2010
ISSN:
0363-6135
Resumen:
Objective: This study describes the vascular inflammation associated with vascular mesenteric remodeling in an experimental model of metabolic syndrome, and the vascular repair found in association with a smaller expression of CD34+/CD309+ cells in the vascular wall of mesenteric arteries. Methods: Wistar Kyoto (WKY) and spontaneously hypertensive (SHR) male rats were distributed in 4 groups (n=8 each): I.WKY; II. FFR: WKY receiving 10% fructose solution as drinking water for 12 weeks; III. SHR; IV. FFHR: SHR receiving 10% fructose solution as drinking water for 12 weeks. Circulating EPCs were stained with fluorescein-conjugated (FITC) CD34 and phycoerythrin-conjugated CD309 antibodies. Flow cytometry analysis was performed by Cell-Quest software. Co-localization of both markers on cell surface was assessed by laser immunofluorescence microscopy. This test was also performed in mesenteric blood vessels samples. Results and Conclusions: FFHR group showed hyperinsulinism, insulinresistance, hyperlipemia, hypertension, oxidate stress and cardiovascular remodeling. Circulating and vascular EPC percentage significantly decreased in FFR (p<0.001) and FFHR (p<0.001), probably decrease vascular repair A greater number of CD34+ cells expressed in the vascular wall probably participates actively in the vascular hypertrophy in this experimental model. Key words: endothelial progenitor cells; fructose-fed hypertensive rats; metabolic syndrome; hypertension; oxidative stress; vascular remodeling.