Human renal adipose tissue from normal and tumor kidney: its influence on renal cell carcinoma

CAMPO-VERDE-ARBOCCO, FIORELLA; SANTIANO, FLAVIA E.; LOPEZ-FONTANA, CONSTANZA M.; PISTONE CREYDT, VIRGINIA; BRUNA, FLAVIA; CONTADOR, DAVID; SASSO, CORINA V.; CALVO, JUAN C; ROMEO, LEONARDO R; GÓMEZ, SILVINA E; ZYLA, LEILA E; CARÓN, RUBÉN W.

Oncotarget

Impact Journals, LLC

Año: 2019 vol. 10 p. 5454 - 5467

1949-2553

Tumor cellscan interact with neighboring adipose tissue. We evaluated components presentin human adipose explants from normal (hRAN) and kidney cancer (hRAT) tissue,and we evaluated the effects of conditioned media (CMs) from hRAN and hRAT onproliferation, adhesion and migration of tumor and non-tumor human renalepithelial cell lines. In addition, we evaluated the expression of AdipoR1, ObR, CD44, vimentin, pERK and pPI3Kon cell lines incubated with CMs. hRAN were obtained from healthy operateddonors, and hRAT from patients who underwent a nephrectomy. hRAT showedincreased levels of versican, leptin and ObR; and decreased levels ofperilipin, adiponectin and AdipoR1, compared to hRAN. Cell lines showed asignificant decrease in cell adhesion and increase in cell migration afterincubation with hRAT-CMs vs. hRAN- or control-CMs. Surprisingly, HK-2,786-O and ACHN cells showed a significant decrease in cell migration afterincubation with hRAN-CMs vs.control-CMs. No difference in proliferation of cell lines was found after 24 or48 h of treatment with CMs. AdipoR1 in ACHNand Caki-1 cells decreasedsignificantly after incubation with hRAT-CMs vs. hRAN-CMs and control-CMs. ObR and CD44 increased in tumor linecells, and vimentin increased in non-tumor cells, after incubation withhRAT-CMs vs. hRAN-CMs andcontrol-CMs. We observed an increase in the expression of pERK and pPI3K inHK-2, 786-O and ACHN, incubated with hRAT-CMs. In conclusion, results showedthat adipose microenvironment can regulate the behavior of tumor and non tumorhuman renal epithelial cells.