IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
artículos
Título:
Make it simple: (SR-A1+TLR7) macrophage targeted nanoarchaeosomes
Autor/es:
CAIMI,AYELEN; VERMEULEN, MONICA; MORILLA, MARIA JOSE; CAIMI,AYELEN; VERMEULEN, MONICA; MORILLA, MARIA JOSE; ALTUBE, MARIA JULIA; DE FARIAS, MARCELO; ROMERO, EDER LILIA; ALTUBE, MARIA JULIA; DE FARIAS, MARCELO; ROMERO, EDER LILIA; PARR, FEDERICO; CARGNELUTTI, DIEGO; VILLARES PORTUGAL, RODRIGO; PARR, FEDERICO; CARGNELUTTI, DIEGO; VILLARES PORTUGAL, RODRIGO
Revista:
Frontiers in bioengineering and biotechnology
Editorial:
Frontiers Media S.A
Referencias:
Año: 2018 vol. 6 p. 163 - 178
ISSN:
2296-4185
Resumen:
Hyperhalophilic archaebacteria exclusively produce sn2,3 diphytanylglycerol diether archaeolipids, unique structures absent in bacteria and eukaryotes. Nanovesicles made of archaeolipids known as nanoarchaeosomes (nanoARC), possess highly stable bilayers, some of them displaying specific targeting ability. Here we hypothesize that nanoARC made from Halorubrum tebenquichense archaebacteria, may constitute efficient carriers for the TLR7 agonist imiquimod (IMQ). NanoARC-IMQ takes advantage of the intense interaction between IMQ and the highly disordered, poorly fluid branched archaeolipid bilayers, rich in archaeol analog of methyl ester of phosphatidylglycerophosphate (PGP-Me), a natural ligand of scavenger receptor A1 (SR-A1). This approach lacks complex manufacture steps required for bilayers labeling, enabling future analytical characterization, batch reproducibility, and adaptation to higher scale production. SR-A1 mediated internalization of particulate material is mostly targeted to macrophages and is extensive because it is not submitted to a negative feedback. A massive and selective intracellular delivery of IMQ may concentrate its effect specifically into the endosomes, where the TLR7 is expressed, magnifying its immunogenicity, at the same time reducing its systemic bioavailability, and therefore it?sin vivo adverse effects. NanoARC-IMQ (600?900 nm diameter oligolamellar vesicles of ∼−43mV Z potential) were heavily loaded with IMQ at ∼44μg IMQ/mg phospholipids [∼20 folds higher than the non-SR-A1 ligand soyPC liposomes loaded with IMQ (LIPO-IMQ)]. In vitro, nanoARC-IMQ induced higher TNF-a and IL-6 secretion by J774A1 macrophages compared to same dose of IMQ and same lipid dose of LIPO-IMQ. In vivo, 3 subcutaneous doses of nanoARC-IMQ+ 10μg total leishmania antigens (TLA) at 50 μg IMQ per Balb/C mice, induced more pronounced DTH response, accompanied by a nearly 2 orders higher antigen-specific systemic IgG titers than IMQ+TLA and LIPO-IMQ. The isotype ratio of nanoARC-IMQ+TLA remained ∼0.5 indicating, the same as IMQ+TLA, a Th2 biased response distinguished by a pronounced increase in antibody titers, without negative effects on splenocytes lymphoproliferation, with a potential CD8+LT induction 10 days after the last dose. Overall, this first approach showed that highly SR-A1 mediated internalization of heavily loaded nanoARC-IMQ, magnified the effect of IMQ on TLR7 expressing macrophages, leading to a more intense in vivo immune response.