IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
artículos
Título:
MODULADORES DE FIBROSIS Y APOPTOSIS ASOCIADOS A LA DISPONIBILIDAD DE ON: EFECTO DE ROSUVASTATINA EN NEFROPATÍA OBSTRUCTIVA NEONATAL.
Autor/es:
LUCIANA MAZZEI; ISABEL MERCEDES GARCÍA; WALTER MANUCHA
Revista:
Revista Bioanalisis
Editorial:
Grupo Bio SRL
Referencias:
Lugar: Mendoza; Año: 2010 vol. 6 p. 20 - 26
ISSN:
1669-8703
Resumen:
  Obstructive nephropathy is a kidney disorder complex to treat due to the severe apoptosis and fibrosis. Previous studies shown that rosuvastatin (Ros), may have potential utility as a therapeutic option in kidney diseases which lead to apoptosis and fibrosis. Objective: to evaluate the possible antifibrotic and antiapoptotic effects of Ros during neonatal unilateral ureteral obstruction (UUO). Materials and Methods: Neonatal rats were surgically obstructed (experimental group) or not (control group), which were Ros treated or not (10 mg/kg per day) during 14 days. Subsequent nephrectomy and processing of the renal cortex to determinate by RT-PCR technique, genes expression of inducible nitric oxide synthase (iNOS), heat shock factor 1 (hsf1), heat shock protein 70  (hsp70), bax, bcl2, wt1, p53, snail, bone morphogenetic protein (bmp7), E-cadherin, transforming growth factor (tgf-â) and tumor necrosis factor (tnf-á). Results: UUO induced fibrosis and apoptosis, while Ros treatment modulated the fibrotic and apoptotic genes pattern and increased the bmp7, caderina E, wt1, p53 and bcl2 expression as well as decreased the profibrotic and proapoptotic genes expression (bax, tnf-á y tgf-â). Our results allow us to suggest that Ros renal protection during UUO is linked to heat shock response and nitric oxide bioavailability interaction, with concomitant decrease in pro apoptotic gene pattern.