IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
artículos
Título:
ROSUVASTATIN PRESERVES RENAL STRUCTURE FOLLOWING UNILATERAL URETERIC OBSTRUCTION IN THE NEONATAL RAT
Autor/es:
MAZZEI LUCIANA JORGELINA; GARCÍA ISABEL MERCEDES; ALTAMIRANO LILIANA; DOCHERTY NEIL G; MANUCHA WALTER
Revista:
AMERICAN JOURNAL OF NEPHROLOGY
Editorial:
KARGER
Referencias:
Lugar: Berlin; Año: 2012 vol. 35 p. 103 - 113
ISSN:
0250-8095
Resumen:
Neonatal ureteric obstruction in rodents can be used as a paradigm for in utero obstruction in humans and a platform for studying the potential of novel therapies for congenital obstructive nephropathy. The present study examined the effect of Rosuvastatin (Ros) on key morphometric and molecular measures of renal injury during neonatal unilateral ureteric obstruction (UUO). Neonatal rats subjected to UUO and controls were treated daily with vehicle or rosuvastatin for 14 days. Quantification of tubular dilatation, glomerular morphometry, tubulointerstitial fibrosis and mRNA expression rates was performed. UUO increased tubular diameter and interstitial volume by 2.7 and 7-fold respectively. Glomerular number and size were reduced by 52% and 33%. Parallel increases in TGF-©¬1 and TNF-¥á mRNA levels and reductions in WT-1, BMP-7 and E-cadherin mRNA levels were shown. Ros attenuated tubular dilatation (33%), interstitial expansion (72%), improve glomerular number and size (30% and 50%), and normalized gene expression levels. Ros treatment attenuated all changes, most notably the increase in interstitial volume. This may be a consequence of the accumulated protective effect of Ros on other parameters. Notably, Ros treatment was unable to completely salvage glomerular development which may have implications for the utility of this type of therapy in neonatal obstruction.