DNA Methylation Index and Methylation Profile of Invasive Ductal Breast Tumors

MARZESE, D.M.; HOON, D.S.; CHONG, K.K.; GAGO, F.E.; OROZCO, J.I.; TELLO, O.M.; VARGAS ROIG, L. M.; ROQUÉ MORENO M

JOURNAL OF MOLECULAR DIAGNOSTICS

AMER SOC INVESTIGATIVE PATHOLOGY, INC

Lugar: Bethesda ; Año: 2012 vol. 14 p. 613 - 622

1525-1578

The goal of this study was to identify alterations in DNA methylation related to invasive ductal carcinomas, evaluating the methylation status of 49 CpG islands (CpG) localized within 34 cancer-involved genes. Our key findings reveal epigenetic alterations which could contribute to a better understanding of the heterogeneity of breast tumorigenesis; and their relationship with previously known prognostic factors. We found that the epigenetic signature is specific for each invasive ductal carcinoma. WT1 and RASSF1 are frequently methylated in IDC. Invasive ductal carcinomas are organized in hierarchical clusters based on the epigenetic information. The methylation status of p73 and RARB is associated with clinic and pathological factors. The concurrent methylation of p73 and RARB is associated with poor prognostic factors. Tumors with poor prognostic factors present a high methylation index. Our study results indicate that MI and methylation of p73 and/or RARB are related to unfavorable prognostic factors of patients with IDCs. Therefore, these epigenetics markers could be included in further studies to improve the molecular diagnosis of patients with invasive ductal carcinomas.