Epigenetic changes induced by fructose on vascular repair mediated endothelial progenitor cells

RENNA NF; LEMBO, C; DIEZ E; MIATELLO RM

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2012

0327-9545

This study describes the vascular inflammation associated with vascular mesenteric remodeling in an experimental model of metabolic syndrome, and the vascular repair found in association with a smaller expression of CD34+/VEGFR2+ cells in the vascular wall of mesenteric arteries. Wistar Kyoto (WKY) and spontaneously hypertensive (SHR) male rats were distributed in 4 groups (n=8 each): I.WKY; II. FFR: WKY receiving 10% fructose solution as drinking water for 12 weeks; III. SHR; IV. FFHR: idem II. Circulating EPCs were stained with fluorescein-conjugated (FITC) CD34 and phycoerythrin-conjugated VEGFR2+ antibodies. Flow cytometry analysis was performed by Cell-Quest software. Co-localization of both markers on cell surface was assessed by laser immunofluorescence microscopy. This test was also performed in mesenteric blood vessels samples. FFHR group showed alterations that characterize the metabolic syndrome. Circulating CD34+/VEGFR2+ cells percentage significantly decreased in FFR (p<0.001) and FFHR (p<0.001). A similar pattern was observed in vascular mesenteric tissue. A greater number of CD34+ cells expressed in the vascular wall probably participates actively in the pathophysiology of vascular remodeling in this experimental model.