IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
artículos
Título:
Virulence profile of Shiga toxin-producing Escherichia coli strains isolated from dogs and cats and comparison by pulsed field gel electrophoresis with isolates from cattle, meat and humans in Argentina.
Autor/es:
BENTANCOR ADRIANA; RUMI MV; CARBONARI C; GERHARD ELIZABETH; LARZÁBAL MARIANO; VILTE DANIEL A; PISTONE CREYDT VIRGINIA; CHINEN ISABEL; IBARRA CRISTINA; CATALDI ANGEL; MERCADO ELSA C
Revista:
VETERINARY MICROBIOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2012 vol. 156 p. 336 - 342
ISSN:
0378-1135
Resumen:
Pets can be reservoirs of Shiga toxin-producing Escherichia coli (STEC) strains. The aim ofthis study was to examine nine strains belonging to several serotypes (O91:H21, O91:H16,O178:H19, O8:H19, O22:H8, O22:HNT, ONT:H8), previously recovered from cats or dogs.To this end, we assessed a set of additional virulence genes (stx2 subtype, subAB, ehxA, eaeand saa), cytotoxic activity, and genetic relationships with strains isolated from cattle,meat and humans using pulsed-field gel electrophoresis (PFGE). Most of the isolates carriedthe stx2 and/or stx2vh-b sequences, while only the O91:H21 isolate presented the mucus-activatable stx2d variant, as confirmed by sequencing the genes of subunits A and B. All thestrains showed cytotoxic activity in cultured cells. One of the two O178:H19, selected forits high level of cytotoxicity in Vero cells, showed the ability to cause functional alterationsin the human colon mucosa in vitro. None of the strains possessed the subAB, eae or saagenes and only the strains belonging to serotype O8:H19 carried the ehxA gene. The isolatesshared 90?100% similarity by PFGE to epidemiologically unrelated strains of thecorresponding serotypes recovered from cattle, meat or humans. Our results demonstratethat dogs and cats may have a role in the infection of humans by STEC, probably serving as avehicle for bovine strains in the cycle of human infection, and thus emphasize the healthrisks for owners and their families.