Garlic components inhibit angiotensin II - induced cell cycle progression and migration: involvement of cell cycle inhibitor p27Kip1 and MAPK

CASTRO, CLAUDIA; GIL LORENZO, ANDREA; GONZALEZ, ADRIANA; CRUZADO, MONTSERRAT

Molecular Nutritiona and Food Research

WILEY-VCH

Año: 2010 vol. 54 p. 781 - 787

1613-4125

Garlic has been used for prevention and treatment of hypertension; however the molecular mechanisms of garlic’s effects remain to be elucidated. In the present study, the mechanisms of the in vitro effect of organosulphur compounds derived from garlic on growth and migration of cultured aortic smooth muscle cells (AoSMC) isolated from spontaneously hypertensive rats (SHR) were investigated. We demonstrated that Allyl methyl sulphide (AMS) and Diallyl sulphide (DAS), inhibited AoSMC angiotensin II-stimulated cell cycle progression and migration. Neither cell viability nor annexin-V binding analysis revealed cytotoxic effects of both organosulphur compounds at the used concentrations. Instead, their inhibitory effects were associated to the prevention of the cell cycle inhibitor p27Kip1 (p27) downregulation and the reduction of ERK 1/2 phosphorilation. When we assessed the antioxidant activity of AMS and DAS we found that both organosulphur compounds inhibited angiotensin II- reactive oxygen species (ROS) generation. Our findings show that AMS and DAS, compounds derivate from garlic, could be effective antioxidants targeted at the arterial remodeling seen in hypertension.