Metodología amigable con el ambiente para la extracción y determinación de contaminantes volátiles en muestras biológicas

MAMMANA, SABRINA B.; BERTON, PAULA; ALTAMIRANO, JORGELINA C.

Congreso; 7° CONGRESO MUNDIAL DE JUVENTUDES CIENTÍFICAS, FUNDACIÓN F.I.S.S. FEDERACIÓN INTERNACIONAL DE SOCIEDADES CIENTÍFICAS; 2014

Polybrominated diphenyl ethers (PBDEs) analysis is of great interest in the toxicological field, since these compounds act as endocrine disruptors even at trace concentrations. Generally, extraction, preconcentration, and cleanup steps are required before their analysis by gas chromatography (GC). Cloud point extraction (CPE), an efficient technique used for extraction and preconcentration, is considered environmental friendly since surfactants are used to replace traditional organic solvents. However, its use prior to gas chromatography (GC) is unfeasible, due to the high viscosity of the surfactants. To overcome this drawback, ultrasound assisted back-extraction technique (UABE) was previously proposed by our group as intermediate step between CPE and GC for PBDEs analysis in samples with simple matrices (environmental waters). In the present work, the methodology is proposed for PBDEs analysis in complex samples (breast milk). The influence of experimental variables on the analytical responses was analyzed and optimized using experimental design (Plackett?Burman and Central Composite Designs). After CPE (5 min, 55 °C, 1% (w/v) Triton-X114) followed by UABE (3 min, 90 µL isooctane), the isooctane phase was injected into GC-ECD (non-polar column DB 5ms, carrier gas: nitrogen) for PBDEs analysis. Using UABE as an intermediate step facilitates the coupling of CPE and GC-ECD, for rapid PBDEs analysis in complex samples such as breast milk samples, using minimum amounts of organic solvents.