THE EFFECT OF SULFATED HYALURONAN ON MONOCYTES/MACROPHAGES IN LUNG CARCINOMA CONTEXT

PAOLO ROSALES ; FIORELLA SPINELLI; DAIANA VITALE; ANTONELLA ICARDI; CHIARA CASSARINI; STEFANO PLUDA; CARLOS GUARESSI; MARIANA G. GARCIA; INA SEVIC; ALANIZ LAURA

Mar del Plata

Congreso; Reunión anual de Sociedades de Biociencias SAIC SAI SAFIS 2020; 2020

SAIC, SAI, SAFIS

Non-small-cell lung carcinoma (NSCLC) is the most frequent lung cancer and has a medium-low survival rate. There is not a successful single treatment, thus it is necessary to search complementary therapeutic option. Sulfated Hyaluronan (sHA) could be used as an adjuvant therapy due to its antiangiogenic and antitumor action in some cancers. Moreover, monocytes/macrophages (Mo/MØ) are critical modulators of the tumor microenvironment and are able to enhance tumor angiogenesis.Aim: evaluate the effect of sHA on Mo/MØ in lung cancer context.M&M: human PBMC cells were isolated by Ficoll-Percoll gradient and were treated with conditioned media (CM) derived from H1299 (NSCLC cell line) plus partial (sHA1) or total sHA (sHA3). Levels of TGF-β1 (total and active) and IL-1β were determined by ELISA as markers of pro or anti-inflammatory Mo/MØ phenotype. Endothelial cell (EC) migratory capacity toward CM of treated Mo/MØ was evaluated in Boyden chamber to evaluate angiogenic modulation. Treated Mo/MØ CM was used to analyze H1299 spheroid formation to analyze antitumoral effect. Statistical analysis: one-way ANOVA and Tukey post-test.Results: Mo/MØ biosynthesis levels of TGF-β1 total and active and IL-1β were non-significant after treatments. We observed a decrease of EC chemotaxis toward CM of treated Mo/MØ with both sHA in lung carcinoma context (p