ALANIZ Laura Daniela
congresos y reuniones científicas
Modulation of mesenchymal stem cells by hyaluronan its sulfated derivatives in osteosarcoma
ANTONELLA ICARDI; INA SEVIC; FIORELLA SPINELLI; DAIANA VITALE; MATIAS VALENZUELA; LUCIANA GUTIERREZ; MARCELA BOLONTRADE; ALANIZ L
Mar del Plata
Congreso; Reunión anual de Sociedades Biocientíficas; 2019
Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad de Farmacología Experimental (SAFE), la Sociedad Argentina de Biología (SAB), la Sociedad Argentina de Protozoología (SAP), la Asociación Argentina de Nanomedicinas (NANOMED-ar), La Asociaci
The treatment and reconstruction of bone in osteosarcoma, after tumor resection, remains a challenge. A promising therapy is the therapeutic use of mesenchymal stem cells (MSCs). Since hyaluronan (HA) is a component of the extracellular matrix, MSCs can interact with HA affecting the differentiation of these cells in therapeutic applications.Aim: To evaluate the therapeutic effect in osteosarcoma of MSCs derived from umbilical cord (hUC-MSCs) treated with HA or its sulfated derivatives (sHA). Also, the role in tissue remodeling and as well as in tumor behavior.Methods: Viability and apoptosis assays by MTS and AnnexinV/IP by flow cytometry were used to determine the dose of HA or sHA to treat hUC-MSCs. Mixed lymphocytes cultures (MLC) were performed to evaluate the effect of HA or sHA on the immunogenicity of hUC-MSCs. The effect of these biomaterials on the bone regenerative capacity of hUC-MSCs was analyzed by differentiation assays. Finally, the effect of conditioned media (CM) of hUC-MSCs treated with HA or sHA on the Saos-2 was analyzed by cellular viability analysis.Results: hUC-MSCs exhibited low immunogenicity under MLC conditions and retained their immune properties after treatment with HA or sHA. HA or sHA reduced differentiation towards adipogenic lineage in vitro, in a dose-dependent manner and associated with the HA sulfation levels. Regarding the capacity for hUC-MSCs osteogenic differentiation, HA treatments showed a tendency to increase in comparison to control. The CM derived from hUC-MSCs had a pro-tumor effect, but the effect decreased when the cells were treated with HA or sHA by decreasing cell viability.Conclusion: The hUC-MSCs are permissive for allogeneic transplantation. However, our results suggest HA and sHA treatments reduced the ability of hUC-MSCs to differentiate towards the adipogenic lineage. Besides, in tumor context favor the tumor behavior of Saos-2, but this effect diminished with HA or sHA treatments.