Preconditioning of human dendritic cells with hyaluronic acid increases their migration capacity in vitro and in vivo.

RIZZO M; PICCIONI F; MALVICINI M; LLOYD R; GARCIA M; ATORRASAGASTI C; BAYO FINA J; FIORE E; ADRIANI O; TERRES M; GONZALEZ-CAMAPAÑA; PODESTA G; ALANIZ L; MAZZOLINI G

Conferencia; 9° Conferencia Internacional de Ácido hialurónico.; 2013

Dendritic cells (DC) are the main cells responsible for initiating and coordinating the immune response to antigens, and it have been used in cancer treatment as therapeutic vaccines. The way in which they are cultured and activated in vitro prior to their use is very heterogeneous as are the results of its clinical use. Once inoculated, the induced immune response depends at least in part on their ability to migrate to secondary lymphoid organs where they can trigger the antitumor response[1]. Hyaluronic acid (HA) is the major component of extracellular matrix and a regulator of several biological processes [2]. Last decade several works have shown that HA is an important factor able to modulate immune response in both physiological and pathological condition [3,4]. We previously have observed that inoculation of low molecular weight (LMW) HA in mice with colon carcinoma was able to stimulate tumor specific immune response [5]. Therefore, we propose the use of LMW HA as a component of the "cocktail" of DC maturation in order to improve its efficiency to generate antitumor immunity. We focus on evaluating changes in migratory capacity of DC using HA. 1. Palucka K, Ueno H, Fay J, Banchereau J (2011) Dendritic cells and immunity against cancer. Journal of Internal Medicine 269: 64-73. 2. Toole BP (2004) Hyaluronan: from extracellular glue to pericellular cue. Nat Rev Cancer 4: 528-539. 3. Jiang D, Liang J, Noble PW (2011) Hyaluronan as an Immune Regulator in Human Diseases. Physiological Reviews 91: 221-264. 4. Alaniz L, Garcia M, Rizzo M, Piccioni F, Mazzolini G (2009) Altered hyaluronan biosynthesis and cancer progression: an immunological perspective. Mini Rev Med Chem 9: 1538-1546. 5. Alaniz L, Rizzo M, Malvicini M, Jaunarena J, Avella D, et al. (2009) Low molecular weight hyaluronan inhibits colorectal carcinoma growth by decreasing tumor cell proliferation and stimulating immune response. Cancer Lett 278: 9-16.