Hyaluronan metabolism is associated with DNA repair genes in breast and colorectal cancer

INA SEVIC; FIORELLA SPINELLI; VITALE DAIANA; ALEJANDRA BRANDONE; LUCIA ROMANO; PAULA GIANNONI; CAROLINA CRISTINA; MARCELA BOLONTRADE; ALANIZ LAURA

Biomedicines

MDPI

Año: 2020

2227-9059

Large numbers of cancer patients lack access to quality diagnosis and treatment in underdeveloped countries due to low-income. Here we use qPCR as one of the cheapest and easiest methods for the screening of potential markers in breast and colorectal cancer. We compared mRNA levels of HA metabolism members and BRCA genes, known to be involved in the tumoral process, between tumor and non-tumor adjacent tissue and its correlation with previously proposed biomarkers (ER, PR, HER2 and KI67) in order to assess their value as a potential biomarker. We show alteration in HA metabolism in colorectal but not breast cancer. Besides, we found a decrease in HYAL1 levels in breast but not colorectal cancer. We also show lower HA levels in tumor compared with normal tissue that could indicate a possible influence of tumor on its surrounding ?normal? tissue. In both, breast and colorectal cancer, CD44 and BRCA2 showed a strong positive correlation. Our results show first indicators that qPCR of the analyzed genes could be used as an easy and low cost procedure for the evaluation of molecular markers we propose here