STUDY OF THE VIABILITY OF TRITRICHOMONAS FETUS IN BOVINE CERVICAL FLUID

FRANCO PARADISO; RAMIREZ MARIA ROSANA; MORERO MARIANA; OYHENART JORGE; ESTEFANIA BRUNO; FUCHS LUMILA

Congreso; Reunión Anual de las Sociedades de Investigación Clínica (SAIC), Farmacología Experimental (SAFE), Biología (SAB), Portozoología (SAP); 2020

Bovine trichomonosis is a venereal disease caused by the protozoan Tritrichomonas foetus and is very common in countries whereextensive husbandry and natural service are practiced. In the bull,the presence of the parasite is asymptomatic and tends to becomechronic in the adult animal. Infection in females occurs naturallyduring heat and usually manifests as failed services and repetitionof heat. In pregnant females, the protozoan can reach the uterusand invade the embryo tissues. The multiplication of T. foetus mustguarantee its persistence until a new estrus and the infection of themale. For the reasons mentioned, in this work the ability of T. foetus to grow in vitro in cervical mucus (MCV) of females at differentstages of the estrous cycle was evaluated. For this, the degree ofcrystallization (fern formation) and the cellularity of each cervical fluid were used in the work methodology in order to infer the momentof the estrous cycle that allows the multiplication of the protozoan.The data were analyzed by ANOVA, using the Graph Pad Prismversion 5.01 program. The preliminary data obtained show that T.foetus showed positive growth during estrus. In contrast, no growthwas observed in the right-hander. The results allow us to concludethat it is hypothesized that the protozoan in a natural infection mustmodify the environment to persist in the female reproductive tractthroughout the entire estrous cycle.