INCITAP   20787
INSTITUTO DE CIENCIAS DE LA TIERRA Y AMBIENTALES DE LA PAMPA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Fungal diglycosidases
Autor/es:
MICHAEL KOTIK; LAURA S. MAZZAFERRO; KATE BRODSKY; VLADIMIR KREN; GISELA WEIZ; JAVIER D. BRECCIA
Lugar:
Budapest
Reunión:
Simposio; Biotrans 2017; 2017
Resumen:
Diglycosidases are rather rare enzymes and occur in some bacteria,[1] fungi[2,3] and plants. They hydrolyze the heterosidic linkage of diglycoconjugates such as rutin or hesperidin, generating a disaccharide and the corresponding aglycon as products. Some diglycosidases exhibit transglycosylation activities, which provide access to various novel diglycoconjugates (Figure 1). We report on the cloning of several diglycosidase-encoding genes from three fungal strains: Mucor circinelloides, Penicillium chrysogenum and Acremonium sp. We succeeded in heterologously expressing some of these diglycosidases in active form using Escherichia coli or Pichia pastoris as a host. This enabled us to characterize these enzymes in terms of their substrate specificities and transglycosylation activities.