Isolation in alginate microcapsules of the pathogen Tritrichomonas foetus, to evaluate the tricomonicidal activity of plant extracts.

OYHENART, JORGE; RAMIREZ, ROSANA

Foz do Iguacu

Congreso; 41ªRASBQ; 2018

SBQ

Área: QPNInscrição: 00070Isolation in alginate microcapsules of the pathogen Tritrichomonas foetus, to evaluate the tricomonicidal activity of plant extracts. Maria R. Ramirez (PQ),1* Jorge A. Oyhenart (PQ) 2.mr.ramirez@conicet.gov.ar1Department of Biological Sciences, Faculty of Biochemistry and Cs. Biological-UNL-CONICET-Santa Fe; 2Department Faculty of Exact and Natural Sciences-UNLPam-INCITAP - CONICET Santa Rosa, La Pampa, Argentina.Key words: Sodium alginate, Bovine trichomonosis, Encapsulation, Plant extracts. HighlightsThe flagellated protozoan parasite Tritrichomonas foetus is a pathogen that causes bovine and feline trichomonosis. Bovine trichomonosis is a venereal disease that leads to reproductive failure in infected animals, resulting in economic loss in beef-producing areas where open range management and natural breeding are practiced. T. foetus has a simple life cycle that consists of a trophozoitic form, that under stress, the trophozoite takes on an endoflagellar form, called a pseudocyst. The pseudocyst is a reversible form and present a distinct mitotic mechanism, known as the budding. Despite its veterinary importance, the use of these microorganisms as a experimental model is restricted by the difficulty that their culture presents for the laboratory. On the other hand, the technique of encapsulation with sodium alginate allows the isolation of these microorganisms in an anaerobic environment, the recovery and the obtaining of colonies from a single cell. In this way, it allows direct use, in order to evaluate plant extracts, using the Tritrichomonas fetus parasite as an experimental model.Resumo/AbstractTritrichomonas fetus is a flagellated protozoan, agent of bovine trichomoniasis, a sexually transmitted disease. The vaccines developed against the parasite have only allowed to shorten this period of infection; that is why it is necessary to look for new therapeutic options. Alginate allows the release of living cells in an anaerobic environment and the obtaining of colonies from a single cell. Thus, they can be used directly in the laboratory as an experimental model to perform bioactivity tests on plant extracts. Therefore, our objective was to implement the encapsulation technique in pearls with alginate as a potential resource for the isolation, protection and recovery of these microorganisms. For the cell immobilization step, several solutions of sodium alginate were prepared and then T. foetus was added. These mixtures were dropped into the calcium chloride solution, thus forming the beads. Pearls with appropriate chemical and physical characteristics were obtained. The capsules were analyzed by means of the microscope, and later cultivated. By validating the results of the liberation of microorganism, was observed that the developed spheres, will be able to be used for the application of the proposal. In conclusion, this method represents a potential resource for the isolation and cultivation of the T. foetus so that it can be used as an experimental model in the laboratory, to look for new chemical compounds of pharmaceutical interest.References: Oyhenart J et al., 2013 Veterinary Parasitology. 0304-4017.