Changes in Amino Acid Profile in Roots of Glyphosate Resistant and Susceptible Soybean ( Glycine max ) Induced by Foliar Glyphosate Application

MOLDES, CARLOS ALBERTO; CANTARELLI, MIGUEL ANGEL; CAMIÑA, JOSÉ MANUEL; TSAI, SIU MUI; AZEVEDO, RICARDO ANTUNES; MOLDES, CARLOS ALBERTO; CANTARELLI, MIGUEL ANGEL; CAMIÑA, JOSÉ MANUEL; TSAI, SIU MUI; AZEVEDO, RICARDO ANTUNES

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Año: 2017 vol. 65 p. 8823 - 8828

0021-8561

Aminoacid profiles are useful to analyze the responses to glyphosate in susceptibleand resistant soybean lines. Comparisons of profiles for 10 amino acids (Asp,Asn, Glu, Gln, Ser, His, Gly, Thr, Tyr, Leu) by HPLC in soybean roots were performedin two near isogenic pairs (four varieties). Foliar application of glyphosate wasmade to soybean plants after 5 weeks of seeding. Roots of four varieties werecollected at 0 and 72 h after glyphosate application (AGA) for amino acids analysisby HPLC. Univariate analysis showed a significant increase of several aminoacids in susceptible as well as resistant soybean lines; however, amino acidsfrom the major pathways of carbon (C) and nitrogen (N) metabolism, such as Asp,Asn, Glu and Gln, and Ser, increased significantly in susceptible varieties at72 h AGA. Multivariate analysis using principalcomponents analysis (2D-PCA and 3D.PCA) allowed to identified and discriminateddifferent groups based on the soybean genetic origin, showing the amino acidresponses on susceptible and resistant varieties. Based on the results, it ispossible to infer that the increase of Asn, Asp, Glu, Gln, and Ser insusceptible varieties would be related to the deregulation of C and N metabolism,as well as changes in the growth mechanisms regulated by Ser.