INVESTIGADORES
BELLOMIO Augusto
artículos
Título:
Microcin J25 triggers cytochrome c release through irreversible damage of mitochondrial proteins and lipids
Autor/es:
NIKLISON CHIROU MV; DUPUY FG; PENA LB; GALLEGO SM; BARREIRO-ARCOS ML; AVILA C; TORRES-BUGEAU C; DE ARCURI BF; BELLOMIO A; MINAHK CJ; MORERO RD
Revista:
INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND CELLULAR BIOLOGY
Editorial:
Elsevier
Referencias:
Lugar: Amsterdam; Año: 2010 vol. 42 p. 273 - 281
ISSN:
1357-2725
Resumen:
We previously showed that the antimicrobial peptide microcin J25 induced the over-production of reactive oxygen species with the concomitant release of cytochrome c from rat heart mitochondria via the opening of the mitochondrial permeability transition pore. Here, we were able to demonstrate that indeed, as a consequence of the oxidative burst, MccJ25 induces carbonylation of mitochondrial proteins, which may explain the irreversible inhibition of complex III and the partial inhibition of superoxide dismutase and catalase. Moreover, the peptide raised the levels of oxidized membrane lipids, which triggers the release of cytochrome c. From in silico analysis, we hypothesize that microcin would elicit these effects through interaction with heme c1 at mitochondrial complex III. On the other hand, under an excess of l-arginine, MccJ25 caused nitric oxide overproduction with no oxidative damage and a marked inhibition in oxygen consumption. Therefore, a beneficial anti-oxidative activity could be favored by the addition of l-arginine. Conversely, MccJ25 pro-oxidative-apoptotic effect can be unleashed in either an arginine-free medium or by suppressing the nitric oxide synthase activity.