MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition

VINCENT PA; BELLOMIO A; DE ARCURI BF; MORERO RD; FARIAS RN

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Academic Press

Lugar: United States; Año: 2005 vol. 331 p. 549 - 551

0006-291X

Microcin J25 appears to have two intracellular targets: (1) RNA polymerase, which was described in Escherichia coli and Salmonella enterica serovars, and (2) cell respiration in Salmonella enterica serovars. C-terminal glycine amidation of the threaded segment localized in the MccJ25 lariat ring region specifically blocked the RNA-polymerase inhibition, but not the cell respiration inhibition and peptide uptake. These results suggest that different regions of the molecule are responsible for each cellular effect, they are localized far away from the beta-hairpin region and the C-terminal region is an important determinant for RNAP inhibition.