Protein profiling of bovine oocytes in vitro maturated in defined media: a proteomic approach

RIOS G; ALBERIO RH; CESARI A

Puerto Madryn

Congreso; XLVI Reunion Anual SAIB; 2010

The aim of this work was to evaluate the effect of a media with defined composition over bovine oocyte maturation by a proteomic approach. Oocytes were maturated in a media supplemented with r-hFSH and 10% FBS (control) or EGF, hyaluronan and cysteamine (EGF) until they reached the metaphase II stage. Denuded zona pellucida-free oocytes were analyzed by bidimensional electrophoresis and master gels were used to detect differentially expressed proteins. The results showed that 173 spots could be detected, where 68 were present in both treatments, 41 were exclusively found in the control condition and 64 were exclusive for the EGF condition. Among the spots shared by both treatments, 17 showed differential intensities, most of them increasing in the control condition. These results demonstrate an effect of the maturation media over oocyte proteomics. The identification of these proteins should serve as the molecular basis of maturation and a clue for the discovery of maturation markers.