IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Potato nutritional improvement: identification of bioactive phenolics and development of a predictive biosynthetic model
Autor/es:
LANTERI, L.; VILLARREAL, F.
Lugar:
Edición on-line
Reunión:
Congreso; LVI Congreso SAIB y XV Congreso SAMIGE; 2020
Resumen:
Potato(Solanumtuberosum)is a staple food of great nutritional value. The content of phenoliccompounds in consumer cultivars is lower than in Andean ones. Thesecompounds contribute to plant defense and have beneficial effects onhuman health. Potato improvement has been aimed at increasingproductivity and resistance to pests, indetriment of its nutritional quality. The general objective of thisproject is to analyze the metabolism of phenolic compounds withbioactive properties in potatoes, in order to contribute to thedevelopment of predictive tools that assist the future nutritionalimprovement of the species. The specific objectives are 1-Characterize the profiles of phenolic compounds in Andean potatocultivars, 2- Determine the bioactive properties of the compounds:antioxidant capacity, cytotoxic and bactericidal activity in vitro,3- Identify the isoforms of the genes involved in biosynthesis ofbioactive phenolic compounds in Andean cultivars, 4- Develop apredictive model of the metabolic network of phenolic compounds thatallows hierarchization of the metabolic fluxes involved. Thesespecific objectives separately provide valuable data. However, theimportance of the project is centered on the integration of the dataprovided by objectives 1 to 3 that is reflected in objective 4, inestablishing correlations between the different analyzes and in thecomplementation between the techniques (biochemical, molecularbiology and bioinformatics) to be used. Our hypotheses are 1- Ahigher content of bioactive phenolic compounds leads to an increasein beneficial biological activities, 2- The implementation of amathematical model of the pathway of phenolic compounds allows theidentification of key flows involved in the biosynthesis of differentbioactive compounds. The Andean cultivars Santa María, Moradita,Waicha and Chaqueña will be used, due to their contrastingmetabolomic profiles and their multiple biological activities. Tostudy the potato tuber metabolome, an undirected metabolomicsstrategy is proposed by UHPLC-ESI-MS and UHPLC-ESI-MS/MS analysis.Potato extracts or enriched fractions will be tested invitroon experimental systems, models of antioxidant, bactericidal orcytotoxic activity developed in our laboratory.To study the potato tuber transcriptome, the long reads RNA-seqtechnique is proposed due to its high sensitivity and precision onidentification of enzyme variants and isoforms. This systemicapproach is useful to identify key genes associated with theproduction of metabolites of interest and to generate optimizedcommercial potato cultivars that provide agronomic and nutritionalvalue. This project will allow the construction of a model based onrestrictions of metabolic regulation of bioactive phenolic compoundsin potato that will serve as a tool for the nutritional improvementof the species.p { margin-bottom: 0.1in; direction: ltr; color: #000000; line-height: 115%; orphans: 2; widows: 2; background: transparent }p.western { font-family: "Calibri", sans-serif; font-size: 11pt; so-language: es-AR }p.cjk { font-family: "Times New Roman", serif; font-size: 11pt; so-language: zh-CN }p.ctl { font-family: "Times New Roman", serif; font-size: 11pt; so-language: ar-SA }a:link { color: #0000ff; text-decoration: underline }