IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
BEHAVIOR OF A GERMIN-LIKE PROTEASE INHIBITOR IN WHEAT SEEDLINGS UNDER SALT STRESS
Autor/es:
MANSILLA, A.Y.; CONDE, R.D.; SEGARRA, C.I.
Lugar:
Puerto Madryn, Chubut (Argentina)
Reunión:
Congreso; XLVI Reunión anual de SAIB; 2010
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Abiotic stress causes morphological and biochemical changes in plants. Previously, we isolated from the wheat leaf intercellular fluid (IF) a protein belonging to the Germin-like protein family with protease inhibitor activity (GLPI), which takes part in the defense mechanisms against septoriosis. Subsequently, we detected that it also has both superoxide dismutase (SOD) and phosphodiesterase activities. This work evaluated the behavior of GLPI on wheat tolerance against salt stress. For this, wheat seedlings were grown under several NaCl concentrations. The first leaf displayed a normal content of GLPI in FI, but increased in the cell wall. Conversely, GLPI decreased in the FI of the second leaf, but remained unchanged in the cell wall. The activities of both SOD and GLPI accompanied these changes. Also, salt stress did notsignificantly change the size of the first leaf, but caused an increase in the content of both chlorophyll and free proline. In contrast, the size, fresh weight, and chlorophyll content decreased in the second leaf. These results suggest that wheat plants have several mechanisms to provide tolerance to salt stress, including location of GLPI into the cell wall.Supported by CONICET and UNMdP.