IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PL-P22 THE DC1-DOMAIN PROTEIN VLG IS INVOLVED IN ARABIDOPSIS FERTILITY
Autor/es:
FRIK J; D IPPOLITO S; FIOL DF; ARIAS LA; TERRILE MC; LOMBARDO MC; CASALONGUE, CA
Lugar:
Salta
Reunión:
Congreso; LV Annual SAIB Meeting and XIV PABMB Congress; 2019
Resumen:
VACUOLELESS GAMETOPHYTES (VLG) is a DC1 domain containing protein that was first characterized in our laboratory as a necessaryfactor to complete female and male gametophytes development in Arabidopsis. Its subcellular localization at the prevacuolar compartment andthe ability to interact with SNARE proteins suggest a role in the vesicular fusion during vacuolar formation. Vlg expression was also detected inthe vasculature, the filament of mature flowers and root elongation zones, implying a broader role for this protein in sporophyte tissues. Sincevlg homozygous insertional mutants are lethal, we generated transgenic silenced lines encoding an artificial microRNA targeting vlg transcriptswith the aim to explore the loss of VLG function. The phenotypic characterization showed a lower yield in quantity of fruits and number ofseeds per fruit in vlg silenced plants as a result of deficient pollination. This phenotype was caused partially by undehisced anthers suggestingreduced male fertility. In addition, the first flowers on the main stem displayed excessive gynoecium elongation which resulted in stigmaexsertion and thus impaired pollen-stigma interactions. Rosette leaves were also more elongated in silenced plants and the number of lateralroots was higher, suggesting that VLG may mediate processes that regulate organ morphology. We found that vlg promoter activity is modulatedby the hormones auxin and jasmonic acid, both involved in another development and further crossed vlg silenced plants with auxin signalingreporter lines to explore alterations due to diminished VLG levels. Taken together, our results show that VLG participates in the processesmediating fertility and morphogenesis in Arabidopsis. Funded by ANPCyT, Conicet, UNMdP, CIC.