IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Characterization of polyhydroxyalkanoate production by Halomonas titanicae KHS3
Autor/es:
AILÉN N. RODRÍGUEZ; CLAUDIA A. STUDDERT ; VIVIANA P CYRAS; DAVID A. DAMICO ; KARINA HERRERA SEITZ
Lugar:
Paraná, Entre Ríos
Reunión:
Encuentro; Sociedad Argentina de Investigación en Bioquímica y Biología Molecular LIV Reunión Anual; 2018
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Hydrocarbon-degrading strain Halomonas titanicae KHS3 was isolated from contaminated seawater in Mar del Plata harbour. Halomonas species have been described as microorganisms that are able to degrade a variety of xenobiotic compounds and accumulate polyhydroxyalcanoates (PHAs). We have obtained the genomic sequence of H. titanicae KHS3 and found homologues of the phaA and phaC (PHA synthase), phaP (phasin), acetylCoA reductase, phaR (regulatory protein of PHA synthesis) and phaZ (depolymerase) genes. The aim of this work was to evaluate and characterize the ability of H. titanicae KHS3 to produce and accumulate PHA when grown in minimal medium with excess of a single carbon source (glucose 1%). In this condition we observed accumulation of PHA in different points along the growth curve. In order to identify the type of PHA produced by H. titanicae KHS3, analysis of the FTiR, DSC and RMN were conducted and the results indicated that in this growth condition H. titanicae accumulates polyhydroxybutyrate (PHB).The polymer was separated from cells using sodium hypochlorite and its amount was estimated by gravimetric determinations (dry cell and PHA weights) and by spectrophotometric measurements of crotonic acid after acid hydrolysis. Our results indicated that when grown on glucose H. titanicae KHS3 is able to accumulate PHB since early stages of growth, reaching up to 50 - 60 % of dry cell weight. In the stationary phase of growth an approximate yield of 1 g PHB/culture liter was obtained.The determination of properties of the produced PHBHt and its molecular weight are under way.