IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Identification of miRNAs differentially expressed under potassium phosphite (KPhi) treatment in potato
Autor/es:
DALEO, GUSTAVO RAÚL; REY, MARÍA FLORENCIA; FELDMAN, MARIANA LAURA; MACHINANDIARENA, MILAGROS FLORENCIA
Lugar:
Puerto Vallarta
Reunión:
Congreso; XVII National Congress on Plant Biochemistry and Molecular BiologyX Joint Mexico - U. S. Symposium-; 2017
Institución organizadora:
Sociedad Mexicana de Bioquímica
Resumen:
Micro RNAs (miRNAs) are small single strand non-coding RNAs, which regulate gene expression at the post-transcriptional level by translational inhibition or mRNAdegradation on the extent of complementarily between miRNA and mRNA. Potato(Solanum tuberosum L.) is the most important horticultural crop in Argentina. Integrated control of diseases is crucial for this crop, where frequent agrochemical applications, particularly fungicides are carried out. An alternative strategy is based on promoting the induced resistance through the application of compounds environmentally friendly. Among them, phosphites, inorganic salts of phosphorous acid, have been proved to be effective. In this context, we have studied the role of phosphites in disease control management and abiotic stress. However, the mechanisms underlying their effect remains unclear and it has been postulated that miRNAs could be involved in these responses. In order to understand these mechanisms we performed RNA sequencing in potato leaves treated or not with KPhi. We have identified 25 miRNAs that were differentially expressed. Among them, 14 are already annotated in miRBase and 11 are mapped to the potato genome and are potential new miRNAs. The analysis of miRNA predicted targets showed genes related to pathogen resistance proteins, transcription factors and oxidative stress related proteins. The functional analysis of these genes can assist to elucidate the pathways involved in KPhi triggered responses. In addition, expression of such miRNAs can eventually been manipulated in an attempt to improve the quality of the plant. This work provides the first characterization of miRNAs involved in KPhi Induced Resistance pathways.