LA FORMACION DE BIOFILMS DE Pseudomonas syringae SE VE AFECTADA POR UNA PROTEINA TIPO GERMINA

NERCESSIAN, DÉBORA; SALVAT CORREA SILVANA; RODRÍGUEZ SIMÓN, CARLOS; MENDIETA, JULIETA

San miguel de Tucumán

Congreso; XII Congreso Argentino de Microbiología General; 2017

SAMIGE

BIOFILM FORMATION OF Pseudomonas syringae IS AFFECTED BY AGERMIN LIKE PROTEIN (GLPI)LAFORMACION DE BIOFILMS DE Pseudomonassyringae SE VE AFECTADA POR UNA PROTEINA TIPO GERMINA CarlosNorberto Rodriguez Simón; Salvat Correa, Silvana Melisa; Nercessian Débora;Mendieta, JulietaInstitutode Investigaciones Biológicas (IIB), FCEyN- UNMdP- CONICET, Funes 3250 7600 Mardel plata, Argentinajumend@mdp.edu.arBiofilm formation is a necessary step for an efficient surface colonizationby microorganisms. Biofilms are communities of multi-specific cells, which areorganized in cumulus and adhered to each other by extracellular polymers(proteins, polysaccharids, nucleic acids) secreted by themselves. All this   determines the formation of an extremelyirregular film, with pores and inner channels, through which the surrounding fluidscan freely flow.Inside this irregular film, the microorganisms can be specializedand they can adopt biochemical characteristics not observed in planktonic cells.This can explain phenomena like the persister cells after antibioticstreatments, pathogenicity and beneficial interactions between microorganismsand plants. In our laboratory we study a protein purified from extracellular fluidof wheat leaves. This protein was called ?Germin like protease inhibitor? (GLPI)and it has multiple enzymatic activities. Besides, GLPI has a possible role inplant defense response against pathogenic microorganisms. Given the importancethat biofilms formation has for the interaction between plants and microorganisms,we decided to investigate the possible effects of GLPI on this process. Biofilmformation of a phyto-pathogenic bacterium (Pseudomonas syringae) and a halophilic archaea (Halobacteriumsalinarum) was determinated inbatch cultures. Two inert surfaces (glass and polystyrene) were tested and differentGLPI dosses and times of incubation were analyzed. The results indicate that GLPIstimulates almost 100% the biofilm formation in glass by H. salinarum, meanwhile, it inhibits 32% the adherence of P. syringae. Based on this, the nextobjective will be study the effect of GLPI on beneficial bacteria biofilmsgrowing upon Solanum lycopersicum rootsand analyze the mechanism involved in this interaction. Financed by UNMdP/CIC-BA