REVEALING ROLES OF AUXIN IN THE PLANT DEFENSE RESPONSE AGAINST BIOTIC STRESS

D´IPPOLITO, S; FIOL, D; CASALONGUE, C.

San Miguel de Tucumán

Congreso; XLV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009

SAIB

D’ Ippólito S; Fiol D; Casalongué CA Instituto de Investigaciones Biológicas, FCEyN, UNMdP. Mar del Plata, Argentina. E-mail: dippolit@mdp.edu.ar The phytohormone auxin plays a central role in many aspects of plant growth and development. However, recent studies have associated novel auxin functions with plant defense mechanisms. In our laboratory a Solanum tuberosum IAR3 gene (StIAR3) was isolated from a potato cDNA library. StIAR3 shows 78% homology with an A. thaliana IAA-amido-hydrolase (IAR3), an enzyme involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 with an A. thaliana IAA-amido-hydrolase (IAR3), an enzyme involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 isolated from a potato cDNA library. StIAR3 shows 78% homology with an A. thaliana IAA-amido-hydrolase (IAR3), an enzyme involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 with an A. thaliana IAA-amido-hydrolase (IAR3), an enzyme involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 IAR3 gene (StIAR3) was isolated from a potato cDNA library. StIAR3 shows 78% homology with an A. thaliana IAA-amido-hydrolase (IAR3), an enzyme involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 with an A. thaliana IAA-amido-hydrolase (IAR3), an enzyme involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 StIAR3 shows 78% homology with an A. thaliana IAA-amido-hydrolase (IAR3), an enzyme involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 A. thaliana IAA-amido-hydrolase (IAR3), an enzyme involved in the release of active auxin through the hydrolysis of inactive aminocid-IAA conjugates. StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 StIAR3 is differentially induced by Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3 silico expression analysis using microarray data shows that StIAR3 Fusarium eumartii infection in potato tubers. In addition, in silico expression analysis using microarray data shows that StIAR3StIAR3 is up-regulated by Manduca sexta infection and methyl jasmonic treatment in Solanaceae and by Pseudomonas syringae and treatment in Solanaceae and by Pseudomonas syringae and Manduca sexta infection and methyl jasmonic treatment in Solanaceae and by Pseudomonas syringae andPseudomonas syringae and Phytophtora infestans infections in A. thaliana. Thus, we hypothesized that auxin regulation by conjugation/hydrolysis takes part of the plant adaptative defense response against biotic stress. In order to study in deep the IAR3 functions, VIGS (Virus Induced Gene Silincing) strategy was performed. We analyzed the expression of StIAR3 in silenced and non-silenced tobacco plants. Bioassays with fungal elicitors were carried out to evaluate the hypersensitive response. These results will allow us to evaluate the impact of IAA-amido conjugates and auxin balance in the plant defense response to fungal pathogens. Bioassays with fungal elicitors were carried out to evaluate the hypersensitive response. These results will allow us to evaluate the impact of IAA-amido conjugates and auxin balance in the plant defense response to fungal pathogens. hypothesized that auxin regulation by conjugation/hydrolysis takes part of the plant adaptative defense response against biotic stress. In order to study in deep the IAR3 functions, VIGS (Virus Induced Gene Silincing) strategy was performed. We analyzed the expression of StIAR3 in silenced and non-silenced tobacco plants. Bioassays with fungal elicitors were carried out to evaluate the hypersensitive response. These results will allow us to evaluate the impact of IAA-amido conjugates and auxin balance in the plant defense response to fungal pathogens. Bioassays with fungal elicitors were carried out to evaluate the hypersensitive response. These results will allow us to evaluate the impact of IAA-amido conjugates and auxin balance in the plant defense response to fungal pathogens. infections in A. thaliana. Thus, we hypothesized that auxin regulation by conjugation/hydrolysis takes part of the plant adaptative defense response against biotic stress. In order to study in deep the IAR3 functions, VIGS (Virus Induced Gene Silincing) strategy was performed. We analyzed the expression of StIAR3 in silenced and non-silenced tobacco plants. Bioassays with fungal elicitors were carried out to evaluate the hypersensitive response. These results will allow us to evaluate the impact of IAA-amido conjugates and auxin balance in the plant defense response to fungal pathogens. Bioassays with fungal elicitors were carried out to evaluate the hypersensitive response. These results will allow us to evaluate the impact of IAA-amido conjugates and auxin balance in the plant defense response to fungal pathogens. StIAR3 in silenced and non-silenced tobacco plants. Bioassays with fungal elicitors were carried out to evaluate the hypersensitive response. These results will allow us to evaluate the impact of IAA-amido conjugates and auxin balance in the plant defense response to fungal pathogens.