GATA TRANSCRIPTION FACTORS DURING HEAT STRESS IN ARABIDOPSIS THALIANA

COLMAN, S.; ZABALETA EJ.; ROLDAN, J.; CÓRDOBA JP.; DISTEFANO AM.; PAGNUSSAT GC.; FIOL DF.

Córdoba

Congreso; LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016

Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular

High temperature is a major environmental stress that causes plant growth arrest and that greatly reduces the yield and the product quality of crops worldwide. In our lab, we found that heat stress (HS) triggers a cell death pathway that resembles ferroptosis, an iron-dependent type of cell death described first in tumor cells. Ferroptosis can be specifically prevented by treating cells with the iron-chelating agent ciclopiroxolamine (CPX) or with the lipophilic antioxidant ferrostatin-1 (Fer-1). RNA seq experiments and differential expression studies showed that GATA, TCP and AP2/EREBP transcription factor genes are upregulated in plants treated with ferroptosis inhibitors. Here, we examine whether the overexpression of different transcription factor (TF) genes is able to prevent ferroptosis-like cell death in Arabidopsis after HS stress. Transgenic lines overexpressing four different GATA genes are studied and different aspects of development and stress response are shown.