IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
In vitro antioxidant and antiproliferative activities of potato cultivars from the Argentinian Northwest polyphenolic extracts
Autor/es:
BARBINI ML; MARTINEZ M.J.; ANDREU A.B
Lugar:
Mar del Plata
Reunión:
Otro; LXI Reunión de la Sociedad Argentina de Investigación Clínica; 2016
Institución organizadora:
SAIC, SAI, SAFE,
Resumen:
Plant polyphenols are secondary metabolites, studied for their beneficial effects on human health. Potato is an important source of antioxidants for human diet. The anticancer activity of polyphenolic extracts has been largely described. Hepatocellular carcinoma (HCC), one of the most frequent tumors worldwide, is associated to high mortality. Although the availability of treatments, HCC patients remain with poor prognosis. Natural compounds appear as a potential source of new anti-HCC drugs. The objective of this work was to study the polyphenolic composition and the in vitro antioxidant and cytotoxic activities of potato polyphenolic extracts (PPE). Six potato varieties were selected: 5 Andean (CCS1283, CCS1307, CS1418, CL658 and CCS1385) and one industrial variety. PPE characterization was made by spectrophotometric assays and HPLC-DAD analysis and the antioxidant activity was studied by DPPH assay. The cytotoxic analysis was performed on a human hepatocellular carcinoma cell line, by the MTT assay. The results showed that phenolic acids are the main group of compounds in all 6 PPE, showing the pigmented varieties higher levels. The characterization of the PPE composition by HPLC-DAD showed that chlorogenic acid is the main phenolic acid in all the varieties, followed by caffeic acid. Anthocyanidin HPLC-DAD profile resulted completely different in the 3 pigmented varieties. Also, pigmented potatos presented higher antioxidant activities, compared to non-pigmented ones. Only 4 of the studied PPE reduced cell viability 50% cytotoxic concentrations (CC50): CCS1385 CC50= 37.28 µg CGA equiv/mL, CS1418 54.55 µg CGA equiv/mL; CCS1307 66.71 µg CGA equiv/mL, and 71 µg CGA equiv/mL for CL658. A positive significant correlation between antioxidant activity and total phenolic content was found and an inverse correlation between antioxidant, total phenolic content and cytotoxic activity, suggesting that PPE of different polyphenolic compositions can exert anti-HCC activity.