IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Protein malnutrition during the critical developmental stage induces liver injury that can lead to a pre-neoplastic state
Autor/es:
ABALO, ROCÍO; CAMPISANO, SABRINA; CHISARI AN; ECHARTE, EM
Lugar:
North Bethesda
Reunión:
Congreso; American Institute Cancer Research Conference; 2016
Institución organizadora:
American Institute Cancer Research
Resumen:
Individuals who suffer malnutrition during gestation, lactation and childhood have an elevated predisposition to develop metabolic diseases in adulthood.We evaluated the effects of protein malnutrition on liver damage and expression levels of inflammation and oxidative stress markers in adult offspring female rats subjected to different malnutrition schemes.Pregnant rats were fed with a control diet (CD) or a low protein diet (LPD). Female offspring rats received a LPD during gestation, lactation and until they were 120 days of age (MM group), a late LPD that began after 60 days (CM), or a LPD administrated during gestation, lactation and up to 60 days, followed by a CD (MC). The control group received only a CD. On day 120 the liver was dissected out. The ultrastructure was analyzed by TEM and the expression levels of TGF-β, TNF-α and GST-pi 1 were determined by RT-qPCR. Hepatocytes from MM livers showed a large number of lipid vacuoles and the cellular matrix was lost. The level of liver injury was lower in the hepatocytes from MC group, which showed mitochondrial swelling and vacuolation. Hepatocytes in CM group showed the lowest injury and the cytoplasmic matrix was more intact than that in MC group. We found high expression levels of TGF-β and GST-pi 1 (This gene product could be used as marker protein for the detection of carcinogénesis) in the malnourished rats: MM group showed the highest expression of both (p