Evidences of posttranscriptional control during the defense response of potato tubers.

D'IPPÓLITO, SEBASTIÁN; SALCEDO, F.; CASALONGUE, C.; GODOY, V.

Villa Carlos Paz, Cordoba

Congreso; Sociedad Argentina de Investigadores Bioquimicos; 2008

SAIB

One important disease of the potato (Solanum tuberosum) tubers is the dry rot caused by Fusarium species. In our lab,around 500 cDNAs were identified after the differential screening of a potato tuber library inoculated with F. eumartii.Here we report the expression analysis of 358 cDNAs from the collection using DNA macroarray technology. Changesin gene expression of potato tubers wounded or wounded and inoculated with F. eumartii at 0 and 24 hs post-treatmentswere monitored. About 85% of the cDNAs were induced at least two-fold after the treatments. 150 of these cDNAswere only induced by F. eumartii at 24 hs post inoculation. Infected tubers specifically accumulated transcriptsencoding amino acid metabolism enzymes involved in nitrogen recycling and mobilization. The mobilization ofnitrogen might be a strategy used by the fungus to ensure successful colonization. In addition, F. eumartii induceddifferential abundance of transcripts for several proteins involved in RNA processing and export. These genes have notbeen related to the potato defense response and belong to different families of RNA-binding proteins. Three of thesecDNAs showed conserved RNA helicase domains. These results contribute to the identification of new candidategenes that might regulate potato tuber responses against F. eumartii at the posttranscriptional level.Supported by UNMdP, ANPCyT and CONICET