The sesquiterpene botrydial produced by Botrytis cinerea induces phosphatidic acid production

D'AMBROSIO JM; GONORAZKY G; SUELDO DJ; LAMATTINA L; GONZÁLEZ-COLLADO I; LAXALT AM

Mar del Plata

Congreso; LI reunión anual SAIB; 2015

Phosphatidic acid is a lipid second messenger involved in the induction of plant defense responses. It is generated via two distinct enzymatic pathways, either via phospholipase D (PLD) or by the sequential action of phospholipase C and diacylglycerol kinase (PLC/DGK). In tomato (Solanum lycopersicum), phospholipase C enzymes are encoded by a multigene named SlPLC1 ? SlPLC6. Recently we have shown that SlPLC2 silenced plants are less susceptible to the necrotrophic fungus Botrytis cinerea. Botrydial is a phytotoxic sesquiterpene produced by B. cinerea that induces diverse plant defense responses such as production of reactive oxygen species (ROS). The aim of this work was to determine whether botrydial is able to induce PA production using tomato cell suspensions as a model system. We observed that PA production was induced in botrydial-treated tomato cells in a time and dose-dependent manner. Botrydial triggered the formation of PA via PLC/DGK but not via PLD. Responses downstream of botrydial-induced PA were studied. PLC inhibitor U73122 or DGK inhibitor R59022 diminished ROS production induced by botrydial. It can be concluded that PLC/DGK-derived PA is a novel component of the signaling pathway activated by botrydial