IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
HALOARCHAEAL BACTERIORUBERINS IMPROVE CRYOPRESERVED RAM SPERM PARAMETERS
Autor/es:
MARTINEZ, C.; ZALAZAR, L.; CERLETTI, M.; DE CASTRO, R.E; CESARI, A.
Lugar:
Mar del Plata
Reunión:
Congreso; 51 Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB); 2015
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Resumen:
Sperm cryopreservation has a significant impact in the livestock reproductive field. Cryopreserved ram sperm ishighly sensitive to freezing/thawing, thus, both half-life and fertilizing capacity of sperm are reduced. These eventsare associated with higher ion permeability, an increase in ROS production affecting membranes stability, amongothers. It was reported that addition of antioxidants, as vitamins E and C, to freezing extender increases spermmotility and membrane stability after thawing. In humans, carotenoids supplied to diets led to an increase in spermmotility and also ameliorated capacitation when added in vitro. Nevertheless, carotenoids on cryopreservation havenot been evaluated. In our lab, a conditional Lon mutant (HVLON3) was constructed in the haloarchaeon Haloferaxvolcanii, that over-produces bacterioruberins. In this work, we studied the effect of these carotenoids oncryopreserved sperm. Ram sperm was thawed and treated with different concentrations of H. volcaniibacterioruberinextract (BE). Viability, progressive (PM) and total motility (TM) were analyzed. Higher percentages of viable cellswere detected after 2h of treatment. Additionally, 20 μM BE significantly improved both PM and TM throughout thetime evaluated (2h). Our results suggest that addition of H. volcaniibacterioruberins reduces membrane damage andimproves motility of cryopreserved sperm.