IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
INTERPLAY BETWEEN HYDROGEN SULFIDE (H2S), H2O2 AND PHOSPHOLIPASE D (PLD) IN ARABIDOPSIS GUARD CELL SIGNALING.
Autor/es:
SCUFFI, DENISE; LAMATTINA, LORENZO; LAXALT, ANA MARÍA; GARCÍA-MATA, CARLOS
Lugar:
San pedro de Atacama
Reunión:
Workshop; Pan American Plant Membrane Biology Workshop; 2015
Resumen:
Stomatal pore regulation is a key process for carbon and water homeostasis of terrestrial plants. Abscisic acid (ABA), the most studied regulator of stomatal movement, induces stomatal closure through volume changes in guard cells. Among the second messengers that participate in ABA-induced stomatal closure are hydrogen sulfide (H2S), H2O2 and phospholipase D (PLD)-derived phosphatidic acid (PA).H2S is a small gas considered as the third endogenous gasotransmitter in both animals and plants. In Arabidopsis thaliana, H2S is enzymatically produced in the cytosol by the L-cysteine desulfhydrase 1(DES1). In our lab we have shown that H2S induces stomatal closure in epidermal peels from different plant species. Moreover we have demonstrated in Arabidopsis that both PLDα1 and PLDδ isoforms are required for ABA-induced stomatal closure. In this work we demonstrate the relationship between H2S, H2O2 and PLD-derived PA during stomatal closure. Results show that while the DES1 mutants des1-1 and des1-2 show a normal response to H2O2, exogenous addition of 100 µM NaHS, a H2S donor do not induces stomatal closure when epidermal peels from Arabidopsis wild type plants are treated with DPI, an inhibitor of NADPH oxidase activity. H2S-induced stomatal closure is also impaired in epidermal peels of pldα1 and pldδ single mutants. Furthermore, exogenous H2S induces an increase of 40% of PA in leaf disc of Arabidopsis wild type but not in pldα1 and pldδ single mutant plants. All together, the presented data strongly support the relationship among the second messengers H2S, H2O2 and PA during stomatal closure in Arabidopsis thaliana.