Characterization of the expression of two genes coding for DC1-domain-containing proteins under biotic and abiotic stresses in Arabidopsis thaliana

ARIAS, L.; D'IPPÓLITO, SEBASTIÁN; CASALONGUE, C.; PAGNUSSAT G.; FIOL, D

Foz do Iguazú

Congreso; 11th International Congess of Plant Molecular Biology; 2015

Mincyt and Brazilian Genetic Society

A transcriptomic study previously performed in Fusarium eumartii ?infected potato tubers led us to the identification novel genes involved defense responses. Among the newly identified transcripts we focused on a gene coding for an unknown function protein belonging to a populated and largely uncharacterized family of DC1-domain-containing proteins. Two putative functional homologues (At2g17740 and At2g44370) were identified in Arabidopsis thaliana by means of BLAST homology searches and transcriptional in silico studies.In this work, we confirmed the up-regulation of At2g17740 and At2g44370 in Arabidopsis challenged with virulent and avirulent strains of Pseudomonas syringae pv. tomato DC3000, as well as with the elicitor oligochitosan. Additionally different expression pattern of these genes were obtained under developmental stages, plant organs and UV-B radiation stress. We also studied the expression profile of At2g17740 and At2g44370 in promoter:GUS lines under biotic and abiotic stress conditions. The analysis of transgenic lines overexpresing translational fusions with GFP allowed us to identify the subcellular localization of these proteins in plasmatic membrane and endomembrane system. Lastly, several protein interactions were identified by yeast two-hybrid system and confirmed by bimolecular fluorescence complementation assay in order to set a reference for biological function.Summarizing, results presented suggest the participation of At2g17740 and At2g44370 in the regulation of the biotic and abiotic stress responses.