Antiviral activity of andean potato polyphenolic extracts against hepatitis B virus.

MARTINEZ M.J.; ANDREU A. B; BARBINI ML

Mar del Plata

Congreso; XLXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN EN BIOQUÍMICA Y BIOLOGÍA MOLECULAR (SAIB).; 2015

SAIB

Hepatitis B virus (HBV) causes acute and chronic liver infections. 400 million people are chronically infected worldwide. Natural polyphenols are a promising source of new antiviral compounds. The aim is to study the antiHBV activity of andean potato polyphenolic extracts (PPE). PPE were obtained from the andean variety CL658 and HepG2 2.2.15 cells (producing infectious HBV) were used to test antiviral activity. Cells were treated with different PPE concentrations, incubated for 6 d. The maximum non-cytotoxic concentration (MNCC) was determined by MTS assay, trypan blue (TB) and ethidium bromide/acridine orange (EB/AO) stainings. Changes in cellular morphology were studied by phase contrast and electron microscopy. HBsAg was assayed as a measure of viral replication. RESULTS: PPE showed cytotoxicity, dependent on concentration and incubation time. MNCC was determined at 50 µg/mL. The number of apoptotic cells increased at concentrations higher than MNCC. EPP treatment at MNCC did not changed cellular morphology at different incubation times. A dose and time dependent reduction in HBsAg levels was detected, suggesting an inhibition of the viral replication. Antiviral activity is also studied by HBeAg and HBV-DNA quantifications. Molecular mechanisms of action will be determined. CONCLUSION: PPE exert antiHBV activity and is a potential source of new drugs for the treatment of HBV.