IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Antifungal activity of recombinant snakin-1 (SN1r) protein involves membrane permeabilization.
Autor/es:
MUÑOZ, F; ALMASIA, N; PAGANO, M.; HOPP, H; DALEO, G.; VAZQUEZ ROVERE, C; GUEVARA, G.
Lugar:
Villa Carlos Paz, Cordoba
Reunión:
Congreso; XLIVReunión Anual de la SAIB; 2008
Institución organizadora:
Sociedad Argentina de Bioquimica y Biologia Molecular
Resumen:
Snakin1 (SN1) is a cysteine-rich peptide isolated from potato tubers with a MW of 6,922 kDa. Previous reports show that SN1 has antimicrobial activity on fungal and bacterial plant pathogens. In order to analyze in depth the antimicrobial mechanism of action, we have successfully subcloned the SN1 cDNA into pQE70 vector, expressed into M15 cells, induced with 1mM IPTG after 2 h. and  purified in Ni-agarose column. Recombinant SN1 (StSN1r) was able to inhibit germination of F. solani spores and growth of hyphae at the same concentrations reported for SN1wt in vitro. When F. solani hyphae and spores were incubated with different concentrations of StSN1r, membrane permeabilization of these structures was observed, as shown by the uptake of the fluorescent dye SYTOX Green. These results show that, as reported for other plants antimicrobial cysteine-rich peptides, cytotoxic activity of StSN1 involves plasma membrane permeabilization.