DC1-domain-containing-proteins: Functional characterization and their role in plant development

D IPPÓLITO, SEBASTIAN; ARIAS, L.; CASALONGUE, C.; PAGNUSSAT G.; FIOL, D

Rosario

Congreso; L reunion anual de SAIB; 2014

A comprehensive study of genes involved in plant defense responses led us to identify two DC1 domain genes (At2g17740 and At2g44370) whose expression is strongly induced by pathogens and elicitors in Arabidopsis thaliana. The analysis of insertional mutants revealed that these genes are required for normal development of the female gametophyte. In order to functionally characterize these genes we made different expression reporter lines. The analysis of promoter:GUS reporter lines showed low expression levels under normal or biotic stress conditions. Overexpressing lines that were constructed as translational fusions with GFP, allowed us to identify the subcellular localization of these proteins in the plasmatic membrane. Through Bimolecular Fluorescence Complementation (BiFC) assays we determined that these proteins interact with two different transcription factors that participate in the regulation of the defense response to biotic stress