IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cytotoxic activity of Andean potato variety of the Argentinean northwest on human Hepatocarcinoma cells
Autor/es:
MARTINEZ M.J.; ANDREU A. B.; BARBINI L.
Lugar:
Buenos Aires
Reunión:
Congreso; VI International Conference on Polyphenols and Health (ICPH 2013); 2013
Institución organizadora:
Universidad Nacional de Buenos Aires
Resumen:
Hepatocellular carcinoma is one of the most frequent cancers and is associated to high mortality. The research for new anti-cancer drugs is focused on compounds from plant origin. Polyphenols are associated to beneficial effects for human health, including antimicrobial, antineurodegerative and antiproliferative activities. Potato is one of the major sources of polyphenols in the human diet. The aim of this work was to study the cytotoxic activity of Andean potato extracts variety of the Argentinean northwest on human Hepatocarcinoma cells. Methanolic extracts of Andean potato variety (CL658) were obtained. Phenolic acids, anthocyanins and flavan-3-ols were quantified. The cytotoxic activity was studied on HepG2, Hep3B, Huh7 cells. They were incubated with different concentrations of the extracts for 24 h, and cell viability was analyzed by the MTS assay. To analyze if the cytotoxic activity is exerted by apoptosis, it was determined by ethidium bromide/acridine orange staining, DNA laddering and flow cytometer. The concentrations in the extract were: phenolic acids, 9.65 mg chlorogenic acid equiv/mL; anthocyanins, 2.13 mg cyd-3-glu equiv/mL; flavan-3-ols, 0.05 mg catechin equiv/mL. The cytotoxic concentrations 50% and 90% were: HepG2 93ug/mL and 351ug/mL; Hep3B: 140ug/mL and 364ug/mL; Huh7 137ug/mL and 442ug/mL. Andean potato extracts present cytotoxic activity on human hepatocarcinoma cells.