IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Microbial biodiversity of three saltern ponds from La Pampa, Argentina
Autor/es:
DI MEGLIO, L.G.; SANTOS, F.; LÓPEZ, C.; PEÑA, A.; ANTÓN, J.; NERCESSIAN, D.
Lugar:
Mar del Plata
Reunión:
Congreso; VIII congreso de microbiologia general; 2012
Institución organizadora:
Sociedad argentina de microbiologia
Resumen:
In this wok we studied three saltern ponds of La Pampa, argentina (Salitral Negro, La Colorada Grande and Guatraché), originated by the invasion of an ancient sea, million years ago. After sea regression, seawater entrapped in lagoons suffered evaporation, generating salt deposits. These deposits are at the present working mines comercially exploited for the production of salt, but there no exist studies about their microbiological diversity. Indeed, their importance as a source of biological resources (microorganisms, enzymes or other microbial compounds) has not been explored. To face up this study a seasonal sample schedule was performed, and water samples were analized both for microbial diversity and for physicochemical characteristics. The biodiversity eas studied by culturable and non-culturable methodologies. For the first, once pure cultures were obtained, comparison of the sequences of the genes codifying the 16S rRNA subunit was performed. Seven microorganisms were determined, belonging two of them to the Bacteria domain (Salicola sp. and Salinicater ruber) and the other five to the Archaea domain (Haloarcula, Halobcterium and Halorubrum genera). By non-culturable methods, DNA from water samples was analyzed by Denaturing Gradient Gel Electrophoresis (DGGE). A high percentage of the obtained sequences resulted from unknown haloarchaea and bacteria, being the unicellular algae Dunaliella sp. and the nanoflagellated halocafeteria seosiensis the only identified Eukaryotes. Isolated microorganisms were tested for the production of antimicrobial compounds, as a way for studying the ecological relationship between them. By top agar assays in Petri dishes, antagonic behavior was detected between different isolates. It was evidenced by the presence of growth inhibition halos in the sensible strains. For the physicochemical analysis, NaCl content, pH, temperature and ionic compositio were determined. Guatraché saltern showed more different conditions respect to the other two environments. Work has been supported by AECI (Agencia Española de Cooperación Internacional) and CONICET.