Expression analysis of genes potentially involved in induced resistance mediated by phosphite in potato.

FELDMAN ML; MACHINANDIARENA MF; DALEO. G.R.; ANDREU A.B.

San Luis

Congreso; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB),; 2011

Sociedad Argentina de Investigacion en Bioquímica y Biologia Molecular

Phosphite (Phi) compounds have the ability to protect plants against different pathogens. Treatments with Phi could be used within an integrated crop management program as an alternative that would serve to reduce the use of fungicides during the crop cycle. Previous results in our lab, have shown through microarray analysis, that in potato sprouts treated with potassium phophite (KPhi), 26 genes were upregulated when they were compared with non treated ones. Within those we have selected 3 genes (Cullin1, ERD15 & BCYA) that are described to be involved in induced resistance (IR) and we validated them by qRT PCR. The aim of this work was to analyze the expression of these genes in potato leaves treated or not with KPhi, infected or not with Phytophtora infestans (Pi). Semi quantitative RT PCR showed that the 3 genes were expressed only in KPhi treated leaves, non infected with Pi. In order to analyze if Pi infection acts sinergically with KPhi, we quantify their expression 24 and 48 hs after infection, in KPhi treated or non treated leaves. After 24 hs of infection, BCYA and Cullin 1 increased their expression in Pi infected leaves, with respect to the non infected ones, however KPhi treatment did not act sinergically, moreover it was reduced for Cullin 1 gene. ERD15 expression did not change with respect to the non infected leaves, but it increased when KPhi was applied. At 48 hs after infection, their expression decreased in some cases even to lower levels than the non infected ones. These preliminary results could help us to understand the complex network of responses involved in IR mediated by KPhi in potato.