A bioinformatic insight into the Arabidopsis phospholipase C 2 (AtPLC2) interactome

LUCIANA ROBUSCHI, ENZO PERK AND ANA M. LAXALT .

Santa Fe

Congreso; RAFV; 2021

Phosphoinositide-specific phospholipase C (PI-PLC) plays an important role in signal transduction during plant development and in the response to various biotic and abiotic stresses. However, how PI-PLCs are regulated and how they control these processes remains to be fully understood. In Arabidopsis, the PI-PLC gene family is composed of nine members (AtPLC1 to AtPLC9), being AtPLC2 the most abundant isoform. AtPLC2 is involved in plant defense responses, stomatal closure, gametophyte development and embryogenesis and is rapidly phosphorylated following flagellin recognition. To gain insights into PLC-regulators, we characterized the interactome of AtPLC2. TurboID proximity-dependent biotin labeling was used to identify AtPLC2 associated proteins. A total of 167 candidates were enriched in the AtPLC2 interactome from Arabidopsis cell suspension culture. Gene Ontology classification revealed multiple AtPLC2-mediated biological processes including actin-filament-based movement, vesicle-mediated transport, exocytosis, brassinoesteroid mediated signaling pathway, in addition to signal transduction. Regarding molecular functions, AtPLC2-asociated proteins were mainly related to protein kinases. Moreover, pathway analysis showed that regulation pathways such as protein modification, calcium regulation and receptor kinases were significantly enriched. Taken together, this work presents valuable information for the identification and functional elucidation of AtPLC2-associated proteins, and provides a reference to identify critical regulators of AtPLC2-mediated signaling.