IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PHOSPHOLIPASE C IN PLANT STRESS AND DEVELOPMENT
Autor/es:
LAXALT LAXALT A.M., ROBUSCHI L, PERK E., D?AMBROSIO J.M., SCUFFI D., GARCÍA-MATA, C.
Reunión:
Conferencia; SAIB; 2021
Resumen:
Phosphoinositide-specific phospholipase C (PI-PLC) plays an important role in signal transduction during plant development and in the response to various biotic and abiotic stresses. However, how PI-PLCs are regulated and how they control these processes remains to be fully understood. Gene families encode PLC enzymes. The hypothesis is that different PLCs participate in signaling induced by different types stress and during development.In Arabidopsis, the PI-PLC gene family is composed of nine members (AtPLC1 to AtPLC9), being AtPLC2 the most abundant isoform that gets rapidly phosphorylated upon pathogen recognition. We showed that AtPLC2 is involved in plant defense responses, stomatal closure, gametophyte development and embryogenesis. To gain insights into PLC-regulators, we characterized the interactome of AtPLC2 by TurboID proximity-dependent biotin labeling. A total of 167 candidates were enriched. Pathway analysis showed a significantly enriched in protein modification, calcium regulation and receptor kinases.In tomato, the PI-PLC gene family is composed of seven members (SlPLC1 to SlPLC7). Tomato plants transiently silenced in different PLC isoforms showed different susceptibility to pathogens such as Botrytis cinerea, Phytophthora infestans, Cladosporium fulvum, Verticillium dahliae and Pseudomonas syringae. We showed that virus-induced gene silencied (VIGS) SlPLC2- plants have i) reduced reactive oxygen species (ROS) and altered plant defense-related gene expression; ii) reduced susceptibility to Botrytis cinerea and Phytophthora infestans and iii) no changes in susceptibility to Pseudomonas syringae infections compared to non-silenced plants. However, on transiently silenced plants we cannot assay the fitness at the whole plant level. Thus, we generate transgene-free loss-of-function SlPLC2 tomato mutants, by employing the CRISPR/Cas9 technology. Our aim is to generate transgene-free PLC loss-of-function tomato mutants, in order to improve plant resistance to pathogens and to study the role of each PLC on plant stress and development.