- A proteomic approach for the identification of serine protease inhibitors in SP and male duct

ZALAZAR, L.; CLEMENTI, M.; FORNÉS, M.; DE CASTRO, R.E.; CESARI, A.

Puerto Madryn

Congreso; 46 Reunión Anual de la SAIB; 2010

SAIB

The presence of serine-protease inhibitors in seminal plasma (SP) and accessory sexual glands has been reported in several mammals. They play a role in balancing protease activities and are believed to be  important  in  regulating  the  fertilization  process,  acting  as decapacitating  factors. An  important  application  to  improve  the membrane  alterations  in  cryopreserved  sperm  (resembling  the capacitation process) might be the addition of these inhibitors to the sperm  thawing  medium.  The  aim  of  the  present  study  was  to investigate  the  presence  of  serine  protease  inhibitors  in  seminal vesicle  (SV)  and  SP  through  proteomic  analysis  and  to  obtain recombinant  inhibitors.  Bidimensional  electrophoresis  was performed from mouse SV and bovine SP low-Mw protein extracts.