IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Apocynin-induced nitric oxide production confers antioxidant protection in maize leaves
Autor/es:
TOSSI, V.; CASSIA, R.; LAMATTINA L
Revista:
JOURNAL OF PLANT PHYSIOLOGY
Editorial:
ELSEVIER GMBH
Referencias:
Año: 2009 vol. 166 p. 1336 - 1341
ISSN:
0176-1617
Resumen:
The effectofapocyninonnitricoxide(NO)synthesisandoxidativestresswasstudied in corn(Zea mays) seedlings.Aftertreatmentwith100 mM apocynin,strongly increased amountsofNOweredetectedintheleaves.ThisNOproductionwas reduced bymorethan70%byNG-nitro-l-arginine methylester(L-NAME),aNO synthase (NOS)inhibitor,buttherewasnoreductioninNOproductionwhenapocynin was appliedincombinationwithdiphenyleneiodonium(aplantNOXinhibitor).When maize seedlingswereUV-B-irradiated,cellulardamageoccurredandreactiveoxygen species (ROS)werefoundwidelydistributedinchloroplastsandmesophyllcells.Pre- treatment withapocyninandcoincidingNOaccumulationpreventedthisdamage. However,theprotectiveeffectwasavertedbyL-NAMEapplication.Leafdiscsplaced in 1MH2O2 for 24hshowedareductioninchlorophyllcontentthatcouldalsobe avoided byapocynintreatment. Our resultsshowthatapocynininducestheaccumulationofNOinleavesofmaize seedlings throughaNOS-likeactivity,amechanismalternativetoNOXinhibition,and confers anaugmentedtolerancetodifferenttypesofabioticoxidativestress. Indeed, weproposetheuseofapocyninasanalternativeapproachtostudyNO functionality inplants. & 2009 ElsevierGmbH.Allrightsreserved Zea mays) seedlings.Aftertreatmentwith100 mM apocynin,strongly increased amountsofNOweredetectedintheleaves.ThisNOproductionwas reduced bymorethan70%byNG-nitro-l-arginine methylester(L-NAME),aNO synthase (NOS)inhibitor,buttherewasnoreductioninNOproductionwhenapocynin was appliedincombinationwithdiphenyleneiodonium(aplantNOXinhibitor).When maize seedlingswereUV-B-irradiated,cellulardamageoccurredandreactiveoxygen species (ROS)werefoundwidelydistributedinchloroplastsandmesophyllcells.Pre- treatment withapocyninandcoincidingNOaccumulationpreventedthisdamage. However,theprotectiveeffectwasavertedbyL-NAMEapplication.Leafdiscsplaced in 1MH2O2 for 24hshowedareductioninchlorophyllcontentthatcouldalsobe avoided byapocynintreatment. Our resultsshowthatapocynininducestheaccumulationofNOinleavesofmaize seedlings throughaNOS-likeactivity,amechanismalternativetoNOXinhibition,and confers anaugmentedtolerancetodifferenttypesofabioticoxidativestress. Indeed, weproposetheuseofapocyninasanalternativeapproachtostudyNO functionality inplants. & 2009 ElsevierGmbH.Allrightsreserved